Person: ÇELİK, ÖZGE
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ÇELİK
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ÖZGE
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Publication Metadata only Effect of magnetic field on peroxidase activities of soybean tissue culture(DIAGNOSIS PRESS LTD, 67 DONDUKOV BLVD, 1504 SOFIA, BULGARIA, 2007-05) Olgun, Atok; Alikamanoğlu, Sema; Rzakoulieva, A.; ATAK, ÇİMEN; ÇELİK, ÖZGE; TR6653; TR141172In this study, the aim was to determine the effect of magnetic field on peroxidase activities of soybean tissue culture. Shoot tips were put into petri dishes and exposed to a magnetic field for a period of 2.2 and 19.8 s at a magnetic flux of 2.9-4.6 mT. The shoot and root formation rate, fresh weights, chlorophyll quantities, total RNA concentrations and peroxidase activities of regenerated shoots from control and treated shoot tips were determined. While the rate of shoot formation was 28.57% in the control group, this rate was increased to 94.33% and 78.18%, respectively, in the explants that were exposed to a magnetic field for a period of 2.2 and 19.8 s. While the percentage of root formation in controls was 4.76%, this rate increased to 47.17% and 54.54%, respectively, in those that were exposed to a magnetic field at the same periods. When the fresh weights were determined, we found that the fresh weights of plantlets regenerated from treated explants were increased relative to controls. Chlorophyll a, chlorophyll b and total chlorophyll contents increased 21%, 13% and 18%, respectively, relative to control groups at 2.2 s. Peroxidase activity significantly increased in all magnetic field treatments (p<0.05). The total RNA concentration of seedlings regenerated from treatment explants significantly increased relative to controls (p<0.05). The regeneration and plant growth of shoot tips exposed to a magnetic field with a 2.2 s period were positively affected by the MF and increased with respect to controls and the length of time exposed.Publication Embargo The effect of magnetic field on the activity of superoxide dismutase(Haliç University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Fındıkzade 34280, İstanbul-Turkey, 2006) Büyükuslu, Nihal; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987; 109872The effects of magnetic field on superoxide dismutase activity were investigated. All living systems are affected by magnetic field and electromagnetic field in a way of their response systems. Since magnetic field has an impact on biochemical reactions that involve more than one unpaired electron, in our study SOD activity, one of the enzyme responsible for antioxidant system, was measured under magnetic fields using an apparatus explained at material methods. There has been a significant increase of SOD activity when passed 0, 1, 9 and 15 times at 2.9-4.6 mT magnetic field density for 0, 2.2, 19.8 and 33.0 seconds respectively.Publication Metadata only The effects of magnetic field on germination of soybean seeds and on the activity of superoxide dismutase and catalase(Springer, 233 Spring St, New York, Ny 10013 USA, 2007-09) Büyükuslu, Nihal; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987; 109872Publication Metadata only Effects of Magnetic Field on Activity of Superoxide Dismutase and Catalase in Glycine max (L.) Merr. Roots(Hard, Post-Office Box, 10-718 Olsztyn 5, Poland, 2009) Büyükuslu, N.; Rzakoulieva, A.; ÇELİK, ÖZGE; TR113987Under a magnetic field the activities of superoxide dismutase (SOD) in vitro and in vivo and accompanying activities of catalase activity in vivo were investigated in soybean roots. In plant cells a magnetic field creates a stress condition as other environmental stress factors do. To respond to the stress conditions, the occurred reactive oxygen species are scavenged by defense systems. In this study, two enzymes of the defense system, SOD and catalase activities were investigated under magnetic field. Enzyme and soybean seeds exposed to a magnetic field for a period of 2.2, 19.8. and 33s at the magnetic flux of 2.9-4.6 mT. SOD activities data were compared with magnetized enzyme and soybean roots. While the absorbance values of enzyme that passed through the magnetic field with a period of 19.8s for 24 hours were measured and SOD activity was significantly increased. At the same time, magnetic field SOD activity of the soybean roots was increased 21.18% relative to control (P<0.05). After soybean seeds were treated by various magnetic fields and time periods, the activities of superoxide dismutase and catalase were significantly increased (P<0.05) during germination. At the 19.8s for 72 hours, SOD and catalase activities were increased 21.15% and 15.20% relative to control, respectively Thus, it is indicated that the function of defense enzymes in seedlings was intensified due to the treatment of magnetic field. The increases of magnetic field exposure times do not cause linear increases in enzyme activities in vitro and in vivo studies.Publication Embargo Stimulation of Rapid Regeneration by a Magnetic Field in Paulownia Node Cultures(2008) Rzakulieva, Aitekin; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653In this study, the aim was to determine the effect of magnetic fields on regeneration of Paulownia node cultures. Paulownia tomentosa node cultures were used to generate explants and these explants were passed through a 2.9- 4.6-mT magnetic flux density 1 and 9 times at 2.2 and 19.8 seconds, respectively. Chlorophyll quantities, total RNA concentrations of shoots and shoot formation rates from control and treated explants were determined. While the shoot formation rate was 61.9% in the control group, this rate was increased in magnetic field experiments and shoot formation was 82.5% in the explants that were exposed to a magnetic field for a 2.2 second period. However, the regeneration percentage of the explants exposed to a MF for a period of 19.8 s was 45%. Chlorophyll a, chlorophyll b and total chlorophyll contents of the 2.2 s group were increased in comparison to the control group. Total RNA concentrations of seedlings regenerated from treatment explants treated for 2.2 seconds significantly increased in comparison to the control (p<0.05). Our experiments show that the exposure duration to MFs is an important factor for plant tissue. MFs may be used in in vitro regeneration studies rapid and for a short time.