Person: ÇELİK, ÖZGE
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ÇELİK
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ÖZGE
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Publication Metadata only Investigation of Tos-17 transposon mobility in salt stressed rice (Oryza sativa L.) plants by IRAP method(Elsevier Science Bv, Po Box 211, 1000 AE Amsterdam, Netherlands, 2017-08-30) Gündüz, Burcu; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 219257; 113987; 185510; 6653Publication Metadata only Raman spectroscopic and chemometric investigation of lipid-protein ratio contents of soybean mutants(2020) Yılmaz, Ayberk; Kabuk, Hayrunnisa Nur; Kaygısız, Ersin; Fausto, Rui; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; ILDIZ, GÜLCE ÖĞRÜÇSeeds belonging to fourth generation mutants (M-4) of Ataem-7 cultivar (A7) variety and S04-05 (S) breeding line salt-tolerant soybeans were investigated by Raman spectroscopy, complemented by chemometrics methods, in order to evaluate changes induced by mutations in the relative lipid-protein contents, and to find fast, efficient strategies for discrimination of the mutants and the control groups based on their Raman spectra. It was concluded that gamma irradiation caused an increase in the lipid to protein ratio of the studied Ataem-7 variety mutants, while it led to a decrease of this ratio in the investigated S04-05 breeding line mutants. These results were found to be in agreement with data obtained by reflectance spectrum analysis of the seeds in the full ultraviolet to near-infrared spectral region and suggest the possibility of developing strategies where gamma irradiation can be used as a tool to improve mutant soybean plants targeted to different applications, either enriched in proteins or in lipids. Ward's clustering and principal component analysis showed a clear discrimination between mutants and controls and, in the case of the studied S-type species, discrimination between the different mutants. The grouping scheme is also found to be in agreement with the compositional information extracted from the analysis of the lipid-protein contents of the different samples.Publication Metadata only Biotic Stress-Tolerant Plants Through Small Rna Technology(Elsevier, 2020) ÇELİK, ÖZGE; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMENWith an increasing population around the world, the rapid loss of agricultural fields shows us the urgent importance of finding solutions to develop agricultural productivity. Besides industrialization, several effectors such as abiotic and biotic factors cause losses in crop productivity. Biotic factors threaten production and transportation of products worldwide. Bacteria, fungi, viruses, and oomycetes cause losses both pre- and postharvest. It is crucial to improve biotic stress-tolerant plants to overcome reduction in plant productivity. For many years, researchers focused on understanding plant defense mechanisms. Anatomical, physiological, and molecular adaptive mechanisms were investigated for several plant species. Besides the innate immunity mechanisms, including cross-talk, among the phytohormones to manage appropriate defense mechanisms against pathogens, new generation strategies in crop improvements are widely used in plant biotechnology. Although conventional breeding has importance in breeding new varieties, new technologies increase the possibility of success. Recently, new combinations of technologies have been proven effective to develop new cultivars. Small RNA technology is one of the developing crop improvement technologies relying on regulating specific genes together with their sequence identity. In this chapter, we focus on the small RNA technologies used in improvement of biotic stress-tolerant plants.Publication Metadata only Epigenetic analysis of WRKY Transcription Factor Genes in Salt Stressed Rice (Oryza sativa L.) plants(Elsevier, 2018) MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 219257; 185510; 6653It is important for plants to survive when they are subjected to environmental stress factors under different combinations in nature. In such conditions, epigenetic modifications such as cytosine methylations have a regulatory role in adaptive responses to changing environment. WRKY transcription factor family is one of the largest families managing regulation of various processes in plants. Although, the WRKYs are known to play important regulatory role in stress tolerance, the exact mechanisms underlying for regulation of each member under different conditions are not clarrified yet. Among WRKY family members, WRKY50 and WRKY72 were reported to be differentially regulated under salt stress. In this study, we compared the level of cytosin methylation patterns of both WRKY transcription factors under different salt concentration treated Osmancık-97 rice variety (Oryza sativa L.) seedlings. DNA methylation status of both genes in leaf tissues of NaCl stressed (0, 30 mM, 90 mM, 150 mM and 210 mM) rice plants were assessed by bisulfite sequencing. The sequencing reads were mapped according to Oryza sativa genome (v.7). Principal Component Analysis and the Fisher’s exact test were performed to compare the levels of methylated cytosines in CpG in the samples. Differentially methylated cytosines were identified and comparatively analyzed by methylKit. A total of 155 MetCs positions in the CpG context were detected comparing all libraries. 72 methylated cytosines correspond to WRKY50 sequence and 83 methylated cytosines were detected in the WRKY72 region. The methylation levels of the WRKY genes were found in negative correlation with gene expression at 90 and 150 mM NaCl treatments, however at 210 mM concentration the data indicate more complex status of the relation.Publication Metadata only Lipid/Protein Ratio Contents of Soybean Mutants: A Raman Spectroscopic and Chemometrics Investigation(2018) Kabuk, Hayrunnisa; Kaygısız, Ersin; Rui, Fausto; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; ILDIZ, GÜLCE ÖĞRÜÇ; 107326; 113987; 6653; 175748; 185510; 219257Publication Metadata only Composition analysis of soybean salt resistant mutants by raman spectroscopy and chemometrics(2019) Yılmaz, Ayberk; Kabuk, Hayrunnisa Nur; Kaygısız, Ersin; Fausto, Rui; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; ILDIZ, GÜLCE ÖĞRÜÇ; 107326; 6653; 175748; 185510; 219257; 113987Soya seeds belonging to fourth generation mutants (MPublication Metadata only Inducing salt tolerance in rice (Oryza sativa L.) varieties by gamma radiation(Elsevier Science Bv, Po Box 211, 1000 AE Amsterdam, Netherlands, 2017-08-30) MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 185510; 113987; 219257; 6653Publication Metadata only Two-dimensional protein profiles of drought tolerant tomato (Solanum lycopersicum) mutants improved by gamma radiation(2017-08-30) Topçuoğlu, Burak; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 185510; 113987; 219257; 6653Publication Metadata only Magnetic field induced production of anti-cancer agent vincristine from Madagascar Periwinkle (Catharanthus roseus) plant(Elsevier Science Bv, Po Box 211, 1000 AE Amsterdam, Netherlands, 2017-08-30) Taş, Meriç; Avcılar, Ceren; MERİÇ, SİNAN; AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 219257; 185510; 6653